Dr. Fernando Alferez


Peer Reviewed:

Cronje, P., Zacarias, L., Alférez, F. Susceptibility to postharvest peel pitting in Citrus fruits as related to albedo thickness, water status, and phospholipase activity.Postharvest Biology and Technology (2017), 123:77-82.

Lafuente, M.T., Alférez, F. Evaluation of LED blue light for the control of Penicillium digitatum and Penicillium italicum strains. JOURNAL: Photochemistry and Photobiology (2015), 91: 1412-1421. (Featured article in the November/December 2015 issue).

Lafuente, M.T., Alférez, F, Romero P. Postharvest ethylene treatment reduces quality loss of stored mature sweet orange ‘Navelate’. Acta Horticulturae (2015), 1065: 1507-1513.

Romero P., Gandía M., Alférez, F*. Postharvest water stress leading to peel disorders in citrus fruit involves regulation of phospholipases by ABA. Acta Horticulturae (2015), 1065: 1515-1519.

Lafuente, M.T., Alférez, F., Romero, P. Postharvest ethylene conditioning as a tool to reduce quality loss of stored mature sweet oranges. Postharvest Biology and Technology (2014), 94:104-111.

Romero, P, Lafuente, M.T., Alférez, F. A transcriptional approach to unravel the connection between phospholipases A2 and D and ABA signal in water stressed citrus fruit and leaves using a fruit specific ABA-deficient mutant. Plant Physiology and Biochemistry (2014), 80: 23-32.

Alférez, F., Zacarias L. Influence of fruit maturity in the susceptibility of Navelina oranges to develop postharvest non-chilling peel pitting. Food Science and Technology International (2014), 20: 183-191.

Romero, P, Gandía, M, Alférez, F. Interplay between ABA and phospholipases A2 and D in the response of citrus fruit to postharvest dehydration. Plant Physiology and Biochemistry (2013) 70: 287-294.

Liao, H-L, Alférez, F, Burns, J.K. Assessment of blue light treatments on citrus postharvest diseases Postharvest Biology and Technology (2013), 81: 81-88.

Alférez, F*., Pozo, L. Roussef, R. and Burns, J. Modification of carotenoid levels by abscission agents and expression of carotenoid biosynthetic genes in Valencia sweet orange. Journal of Agricultural and Food Chemistry (2013) 61: 3082-3089.

Alférez, F*, Burns, J.K. Response to CMNP is under hormonal control and involves phospholipid signaling Acta Horticulturae (2013) 965: 101-104.

AUTHORS:   Romero, P., Rodrigo, M.J.,  Alférez, F., Ballester, A.R. GonzálezCandelas, L., Zacarías, L., Lafuente, M.J. Unraveling molecular responses to moderate water stress in sweet orange (Citrus sinensis L. Osbeck) by using an ABA-deficient mutant. Journal of Experimental Botany (2012) 63: 2753-2767.

M. J. Rodrigo, Alquézar, B., F. Alférez, L. Zacarías  Biochemistry of fruits and fruit products.     Handbook of Fruits and Fruit Processing, 2nd Ed. (Sinha, Barta, Cano, Sidhu and Wu, eds)   Wiley-Blackwell, 2012.

Alferez, F*, Liao, H-L y Burns, J. Light from different wavelengths affects expression of genes encoding phospholipases A2 and D in peel from mandarins at different maturation stage during postharvest storage. Acta Horticulturae (2012) 956: 629-634.

Alferez, F*, Liao, H-L y Burns, J.  Blue light alters infection by Penicillium digitatum in tangerines  Postharvest Biology and Technology (2012) 63: 11-15.

P. Romero, M. J. Rodrigo, F. Alferez, L. Zacarías y M. T. Lafuente. Transcriptomic responses of Navelate orange (Citrus sinensis L. Osbeck) fruit to water stress. Acta Horticulturae (2011) 892: 351-356.

Alférez, F.*, P. Romero, M. Gandía. Inhibiting phospholipase A2 and D activities alters postharvest performance of Citrus fruit. Acta Horticulturae (2010) 877: 781-788.

Alquézar, B., Mesejo, C., Alférez, F., Agustí, M. y Zacarías, L. Morphological and ultrastructural changes in peel of ‘Navelate’ oranges in relation to variations in relative humidity during postharvest storage and development of peel pitting. Postharvest Biology and Technology (2010) 56: 163-170.

Alférez, F., Alquézar, B., Burns, J.K y Zacarías, L. Variation in water, osmotic and turgor potentials during development of postharvest peel pitting in ‘Marsh’ grapefruit. Postharvest Biology and Technology (2010) 56: 44-49.

P. Romero, M. J. Rodrigo, F. Alferez, J. Gadea, L. Zacarías y M. T. Lafuente.  Transcriptomic changes associated with postharvest susceptibility of an ABA deficient mutant of oranges to non-chilling peel pitting      Acta Horticulturae (2010) 877: 1079-1084.

Alférez, F.* y Burns, J. La actividad sPLA2 en cítricos está asociada con la maduración, senescencia y abscisión del fruto. Avances en maduración y postrecolección de frutas y hortalizas, pp 54-61. Ed. Acribia, Spain (2008).

Alférez, F*. Lipid metabolism and signaling during postharvest storage of horticultural crops JOURNAL: Stewart Postharvest Review (2008) 4:1-8.

Alférez, F., Lluch, Y, Burns, J.  Phospholipase A2 and postharvest peel pitting in Citrus fruit. Postharvest Biology and Technology (2008) 49: 69-76.

Alférez, F., Zhong, G. Burns, J. A citrus abscission agent induces anoxia-and senescence-related gene expression and plant decline in Arabidopsis. Journal of Experimental Botany (2007) 58: 2451-2462.

Zacarias, L. y Alférez, F.  Regulation by carbon dioxide of wound-induced ethylene biosynthesis in the peel of citrus fruit. Food Science and Technology International (2007) 13: 497-504.

Alférez, F., Pozo, L. Burns, J. Physiological changes associated with senescence and abscission in mature citrus fruit induced by 5-chloro-3-methyl-4-nitro-1H-pyrazole and ethephon application. Physiologia Plantarum 127: 66-73 (2006).

Alférez, F., Sala, J.M., Sánchez-Ballesta, M.T., Mulas, M., Lafuente, M.T., Zacarías, L. A comparative study of the postharvest performance of an ABA deficient mutant of oranges: I. Physiological and quality aspects. Postharvest Biology and Technology, 37:222-231 (2005). 

Sala, J.M., Zacarías, L., Sánchez-Ballesta, M.T., Alférez, F., Mulas, M. and Lafuente M.T.  A comparative study of the postharvest performance of an ABA deficient mutant of oranges: II. Antioxidant enzymatic system and phenylalanine ammonia-lyase in nonchilling and chilling rind disorders. Postharvest Biology and Technology, 37:232-240 (2005).

Yuan, R., Alferez, F., Kostenyuk, I., Singh S., Syvertsen, J. and Burns, J.  Partial defoliation can decrease average leaf size but has little effect on orange tree growth, fruit yield and juice quality.  Hortscience, 40: 2011-2015 (2005).

Alferez, F, Zacarias, L., and Burns, J.  Low relative humidity at harvest and before storage at high humidity influence the severity of postharvest peel pitting in Citrus fruit.  J Am Soc Hort Sci, 130: 225-231 (2005).

Alferez, F; Singh, S, Umbach A, Hockema, B  and Burns, J.  Citrus abscission and Arabidopsis plant decline in response to 5-chloro3methyl-4-nitro-1H-pyrazole are mediated by reduction in ATP and activation of lipid signaling. Plant Cell & Environment, 28: 1436-1449 (2005).

Pozo, L., Yuan, R., Kostenyuk, I., Alferez, F., Zhong, G and Burns, J. Combined applications of 1-methylcyclopropene and ethephon reduce defoliation but have no effects on mature fruit abscission in citrus.  J. Am Soc. Hort. Sci , 129: 473-478 (2004).

Alferez, F and Burns, J.  Postharvest peel pitting at non-chilling temperatures in grapefruit is promoted by changes from low to high relative humidity during storage.  Postharvest Biology and Technology. 32, 79-87 (2004).

Rodrigo, M.J; Marcos, J.M.; Alférez, F.; Mallent, D. and Zacarías, L. Characterization of Pinalate, a novel Citrus sinensis variety with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content. Journal of Experimental Botany, 54: 727-738 (2003). (Selected for Journal cover image)

Alférez, F.; Agustí, M. and Zacarías, L.  Postharvest rind staining in Navel oranges is aggravated by changes in storage relative humidity. Effect on respiration, ethylene production and water potential. Postharvest Biology and Technology, 28: 143-152 (2003).

Agustí, M., Almela, V., Juan, M., Alférez, F.; Tadeo, F.R. and Zacarías, L.  Histological and physiological characterization of rind breakdown of Navelate sweet orange. Annals of Botany, 88, 415-422 (2001).

Alférez, F. and Zacarías, L. Postharvest pitting in Navel oranges at non-chilling temperature: influence of relative humidity. Acta Horticulturae, 553: 307-308 (2001)

Zacarías, L.; Alférez, F.; Mallent, D. and Lafuente, M.T. Understanding the role of plant hormones during development and maturation of Citrus fruits through the use of mutants. Acta Horticulturae, 463, 89-96 (1997) 


Extension publications:

Romero, P, Lafuente, M.T. and Alférez, F. Cambios en el transcriptoma de frutos de naranja Navelate en respuesta a estrés hídrico generado por cambios bruscos de humedad durante la postcosecha. HORTICULTURA, 2015.

Lafuente, M.T., Alférez, F. and Romero, P. El acondicionamiento de frutos maduros con etileno reduce la pérdida de calidad durante el almacenamiento de naranjas Navelate. Levante Agrícola, 417 (ABSTRACT, 2014)

Romero, P, Gandía, M. and Alférez, F. El estrés hídrico causante de desórdenes fisiológicos en la piel de los frutos cítricos durante la postcosecha implica la regulación de diferentes fosfolipasas por ABA. Levante Agrícola, 417 (ABSTRACT, 2014)

Alférez, F., Liao, H-L and Burns, J.El tratamiento con luz azul durante la postcosecha afecta a la susceptibilidad de los frutos cítricos a la infección alterando la biosíntesis de oxilipinas. Levante Agrícola, 417 (ABSTRACT, 2014)

Sala, J.M., Zacarías, L., Sánchez-Ballesta, M.T., Alférez, F., Mulas, M. and Lafuente, M.T. Implicacion del sistema enzimatico antioxidante y de la fenilalanina amonio-liasa en el desarrollo de alteraciones postcosecha de los frutos citricos. Levante Agrícola (2004)

Lafuente, M.T., Alférez, F., Sánchez-Ballesta, M.T., Sala, J., Mulas, M. and Zacarias, L. Alteraciones fisiológicas durante la postcosecha de frutos cítricos: tratamientos de control y mecanismos implicados Levante Agrícola, 355, 128-133 (2001)                                  

Zacarías, L., Alférez, F., Mallent, D. and Lafuente, M.T.Estudios de la maduración del fruto y sus mecanismos de regulación hormonal en mutantes de cítricos. Fruticultura 92, 40-47 (1998)

Assistant Professor, Citrus Horticulturist