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Huanglongbing (HLB) Diagnostic Lab

Welcome to the HLB Diagnostic Lab at UF/IFAS SWFREC in Immokalee. We are a new facility dedicated to the molecular diagnostics of Huanglongbing (HLB), also known as Greening disease of citrus. We are currently accepting suspect HLB citrus samples from growers, researchers and homeowners.

We now test for HLB in psyllids. This new service is available to growers for detection of HLB in psyllids, the insect vector for citrus greening. If you are interested in this service please contact us at (239) 658-3408 and we will be happy to assist you.

Sample Submission Information

Each sample must be accompanied by a completed sample submission form or submitted by e-mail toDr. Ozgur Batuman - University of Florida, Institute of Food and Agricultural Sciences obatuman@ufl.edu. A table format including sample ID, variety, collection date, block, row/tree number, and township/range/section is the preferred format for e-mail submission.

For sampling instructions, please refer to the Procedure for Sampling and Shipping Plant Material.

If you intend to submit numerous samples, please give us a call at 239-658-3400, we will be glad to coordinate the best time to process your samples. We are open Monday through Friday from 8:00 AM to 5:00PM.

Note: Submission of samples to the UF HLB Diagnostic Laboratory constitutes consent for anonymous data pertaining to those samples to be used for research by the University of Florida as seen fit. The Sample Submitter assumes all risks and liabilities associated with sample testing. The UF HLB Diagnostic Laboratory shall not be liable for any damages arising from, related to, or caused by sample testing and/or the use of information generated by sample testing.

We now test for HLB in psyllids. This new service is available to growers for detection of HLB in psyllids, the insect vector for citrus greening. If you are interested in this service please contact us at (239) 658-3408 and we will be happy to assist you.

What happens to samples after they arrive at the HLB Lab?

 HLB Lab - Processing Samples in the HLB Lab

HLB Lab - Processing Samples in the HLB Lab [pdf]

Procedure for Sampling and Shipping Plant Material

Please follow the instructions below to submit citrus samples to be screened for the presence of Candidatus Liberobacter asiaticus, the causal agent of HLB.

  1. Collect leaves with stems intact from live branches with fully expanded leaves. Samples should be collected from symptomatic branches if present. Submit a minimum of 10 leaves. Please do not submit fruit samples.
  2. Place the sample into a re-sealable plastic bag (e.g., Zip-lock), squeeze the air from the bag and seal it.
  3. Assign and record on the bag a unique sample identification number (Grower’s sample ID). Complete a corresponding HLB Sample Submission Form. Forms are available here. Please make sure the Sample Submission Form is complete, providing the exact sample location. We will not process samples lacking sample location and/or client information.
  4. Place the form and the bagged sample into a second resealable bag. Keep samples cool (on ice) until shipment. Do not freeze the samples.
  5. Samples should be submitted in person or shipped overnight either on the day collected or before noon the following day (samples will deteriorate if delayed in transit).
  6. For sampling instructions, please refer to the HLB Lab - Procedure for Sampling and Shipping Plant Material

Please submit samples Monday through Thursday only to:

HLB Lab
2685 SR 29 N
Immokalee, Fl 34142
Phone: (239)658-3400
email: obatuman@ufl.edu

Additional Information

Sample Results

Results will be submitted to the client in a timely manner. Results will be reported as positive, negative or inconclusive: A positive result indicates the presence of Candidatus Liberobacter asiaticus in the sample under our testing conditions. A negative result indicates that Candidatus Liberobacter asiaticus was NOT DETECTED in the sample under our testing conditions, but does not unequivocally indicate that the sample is free of Candidatus Liberobacter asiaticus. If an HLB sample result is negative, resubmission at a later time should be considered. An inconclusive result indicates that another sample should be resubmitted and retested.

Links and Resources

UF/IFAS SWFREC HLB Lab

hlb-lab-500x400
We are currently accepting suspect HLB citrus samples from growers, researchers and homeowners.